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Acquisition of Accurate Data from Intramolecular Quenched Fluorescence Protease Assays
The Intramolecular Quenched Fluorescence (IQF) protease assay utilizes peptide substrates containing donor-quencher pairs that flank the scissile bond. Following protease cleavage, the dequenched donor emission of the product is subsequently measured. Inspection of the IQF literature indicates that...
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| Publié dans: | Anal Biochem |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5390485/ https://ncbi.nlm.nih.gov/pubmed/28119065 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2017.01.020 |
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