siRNA carrying an (E)-vinylphosphonate moiety at the 5΄ end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2

Efficient gene silencing by RNA interference (RNAi) in vivo requires the recognition and binding of the 5΄- phosphate of the guide strand of an siRNA by the Argonaute protein. However, for exogenous siRNAs it is limited by the rapid removal of the 5΄- phosphate of the guide strand by metabolic enzym...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Elkayam, Elad, Parmar, Rubina, Brown, Christopher R., Willoughby, Jennifer L., Theile, Christopher S., Manoharan, Muthiah, Joshua-Tor, Leemor
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2017
Soggetti:
RNA
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5389677/
https://ncbi.nlm.nih.gov/pubmed/27903888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw1171
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