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Identification of an Amino Acid Position That Determines the Substrate Range of Integral Membrane Alkane Hydroxylases

Selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (AHs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. First, substrate range mutants of the Pseudomonas putida GPo1 and Alcanivo...

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Bibliografische gegevens
Hoofdauteurs: van Beilen, Jan B., Smits, Theo H. M., Roos, Franz F., Brunner, Tobias, Balada, Stefanie B., Röthlisberger, Martina, Witholt, Bernard
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 2005
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC538836/
https://ncbi.nlm.nih.gov/pubmed/15601691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.1.85-91.2005
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