Identification of an Amino Acid Position That Determines the Substrate Range of Integral Membrane Alkane Hydroxylases

Selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (AHs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. First, substrate range mutants of the Pseudomonas putida GPo1 and Alcanivo...

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Autors principals: van Beilen, Jan B., Smits, Theo H. M., Roos, Franz F., Brunner, Tobias, Balada, Stefanie B., Röthlisberger, Martina, Witholt, Bernard
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Enzymes and Proteins
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC538836/
https://ncbi.nlm.nih.gov/pubmed/15601691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.1.85-91.2005
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