Identification of an Amino Acid Position That Determines the Substrate Range of Integral Membrane Alkane Hydroxylases

Selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (AHs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. First, substrate range mutants of the Pseudomonas putida GPo1 and Alcanivo...

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Publicado en:J Bacteriol
Autores principales: van Beilen, Jan B., Smits, Theo H. M., Roos, Franz F., Brunner, Tobias, Balada, Stefanie B., Röthlisberger, Martina, Witholt, Bernard
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2005
Materias:
Enzymes and Proteins
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC538836/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15601691/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.187.1.85-91.2005
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