Identification of an Amino Acid Position That Determines the Substrate Range of Integral Membrane Alkane Hydroxylases

Selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (AHs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. First, substrate range mutants of the Pseudomonas putida GPo1 and Alcanivo...

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Detalhes bibliográficos
Main Authors: van Beilen, Jan B., Smits, Theo H. M., Roos, Franz F., Brunner, Tobias, Balada, Stefanie B., Röthlisberger, Martina, Witholt, Bernard
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Enzymes and Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC538836/
https://ncbi.nlm.nih.gov/pubmed/15601691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.1.85-91.2005
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