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Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated, homology-independent knock-in system
The CRISPR/Cas9 system has revolutionized genome editing in virtually all organisms. Although the CRISPR/Cas9 system enables the targeted cleavage of genomic DNA, its use for gene knock-in remains challenging because levels of homologous recombination activity vary among various cells. In contrast,...
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| Udgivet i: | Mol Biol Cell |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The American Society for Cell Biology
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5385939/ https://ncbi.nlm.nih.gov/pubmed/28179459 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E17-01-0051 |
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