Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo.
Three major difficulties have hindered the general application of in vivo recombination techniques to library screening: (i) the original selection could not be applied to libraries prepared in phage vectors lacking amber mutations, (ii) nonirradiated packaging extracts gave high backgrounds even wh...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1990
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53855/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2183225/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.8.3166 |
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