Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo.

Three major difficulties have hindered the general application of in vivo recombination techniques to library screening: (i) the original selection could not be applied to libraries prepared in phage vectors lacking amber mutations, (ii) nonirradiated packaging extracts gave high backgrounds even wh...

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Bibliographic Details
Main Authors: Kurnit, D M, Seed, B
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC53855/
https://ncbi.nlm.nih.gov/pubmed/2183225
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