Rapid cloning of HLA-A,B cDNA by using the polymerase chain reaction: frequency and nature of errors produced in amplification.

A method for cloning full-length HLA-A,B cDNA (1.1 kilobases) by using the polymerase chain reaction (PCR) is described. Six HLA-A,B alleles (HLA-A2, -A25, -B7, -B37, -B51, and -B57) were cloned, and their structures were determined. Multiple PCR clones for each allele were sequenced to obtain both...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Ennis, P D, Zemmour, J, Salter, R D, Parham, P
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1990
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53785/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2320591/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.7.2833
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