Rapid cloning of HLA-A,B cDNA by using the polymerase chain reaction: frequency and nature of errors produced in amplification.
A method for cloning full-length HLA-A,B cDNA (1.1 kilobases) by using the polymerase chain reaction (PCR) is described. Six HLA-A,B alleles (HLA-A2, -A25, -B7, -B37, -B51, and -B57) were cloned, and their structures were determined. Multiple PCR clones for each allele were sequenced to obtain both...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1990
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53785/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2320591/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.7.2833 |
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