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Mechanism of ribosome rescue by ArfA and RF2

ArfA rescues ribosomes stalled on truncated mRNAs by recruiting release factor RF2, which normally binds stop codons to catalyze peptide release. We report two 3.2 Å resolution cryo-EM structures – determined from a single sample – of the 70S ribosome with ArfA•RF2 in the A site. In both states, the...

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Bibliographische Detailangaben
Veröffentlicht in:eLife
Hauptverfasser: Demo, Gabriel, Svidritskiy, Egor, Madireddy, Rohini, Diaz-Avalos, Ruben, Grant, Timothy, Grigorieff, Nikolaus, Sousa, Duncan, Korostelev, Andrei A
Format: Artigo
Sprache:Inglês
Veröffentlicht: eLife Sciences Publications, Ltd 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5378476/
https://ncbi.nlm.nih.gov/pubmed/28300532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.23687
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