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Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts

Reduction of NAD(+) by dehydrogenase enzymes to form NADH is a key component of cellular metabolism. In cellular preparations and isolated mitochondria suspensions, enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been shown to be an effective approach for measuring...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Sci Rep
Päätekijät: Moreno, Angel, Kuzmiak-Glancy, Sarah, Jaimes, Rafael, Kay, Matthew W.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Nature Publishing Group 2017
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5374639/
https://ncbi.nlm.nih.gov/pubmed/28361886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep45744
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