NADH Enzyme-Dependent Fluorescence Recovery after Photobleaching (ED-FRAP): Applications to Enzyme and Mitochondrial Reaction Kinetics, In Vitro

NADH enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) was evaluated for studying enzyme kinetics in vitro and in isolated mitochondria. Mass, optical, and nuclear magnetic resonance spectroscopy data were consistent with the UV NADH photolysis reaction being NADH → NAD(·) + H(+)...

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Detalhes bibliográficos
Main Authors: Joubert, Frederic, Fales, Henry M., Wen, Han, Combs, Christian A., Balaban, Robert S.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2004
Assuntos:
Bioenergetics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1303832/
https://ncbi.nlm.nih.gov/pubmed/14695307
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