Regulated high-level expression of the mannitol permease of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli.
The structural gene (mtlA) of the Escherichia coli phosphoenolpyruvate-dependent mannitol-transport protein (EIImtl) and its upstream promoter region (Pmtl) were subcloned approximately 150 base pairs downstream of a lambda PR promoter on a multicopy mutagenesis/expression vector and used to transfo...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53740/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2181442/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.7.2613 |
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