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Probing Cdc42 polarization dynamics in budding yeast using a biosensor

Cdc42 is a small guanosine triphosphatase (GTPase) that plays a central role in polarity development in diverse cell types. Since the activity of Cdc42 is dynamically controlled in time and space, it is required to develop a biosensor to monitor its activation in vivo. In this chapter, we describe t...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Okada, Satoshi, Lee, Mid Eum, Bi, Erfei, Park, Hay-Oak
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5367485/
https://ncbi.nlm.nih.gov/pubmed/28336063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.01.011
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