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Probing Cdc42 polarization dynamics in budding yeast using a biosensor
Cdc42 is a small guanosine triphosphatase (GTPase) that plays a central role in polarity development in diverse cell types. Since the activity of Cdc42 is dynamically controlled in time and space, it is required to develop a biosensor to monitor its activation in vivo. In this chapter, we describe t...
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| Veröffentlicht in: | Methods Enzymol |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5367485/ https://ncbi.nlm.nih.gov/pubmed/28336063 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.01.011 |
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