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Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

BACKGROUND: Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medi...

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Pubblicato in:BMC Biotechnol
Autori principali: Orlova, Nadezhda A., Kovnir, Sergey V., Gabibov, Alexandre G., Vorobiev, Ivan I.
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5366130/
https://ncbi.nlm.nih.gov/pubmed/28340620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-017-0353-6
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