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The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Rare arginine codons AGA and AGG affect the heterologous expression of proteins in Eschericha coli. The tRNAs necessary for protein synthesis are scarce in E. coli strain BL21(DE3) pLysS and plentiful in strain BL21(DE3) CodonPlus −RIL. We evaluated in both bacterial strains the effect of these rare...

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Detalhes bibliográficos
Publicado no:Biotechnol Rep (Amst)
Main Authors: Aguirre-López, Beatriz, Cabrera, Nallely, de Gómez-Puyou, Marietta Tuena, Perez-Montfort, Ruy, Gómez-Puyou, Armando
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5361128/
https://ncbi.nlm.nih.gov/pubmed/28352562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2017.01.002
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