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Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

Recombinant chimeric α-amylase (Ba-Gt-amy) has been produced extracellularly in Pichia pastoris under AOX promoter. Clones of P. pastoris with multiple gene copies have been generated by multiple transformations and post-transformational vector amplification, which led to 10.7-fold enhancement in α-...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Parashar, Deepak, Satyanarayana, Tulasi
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5360700/
https://ncbi.nlm.nih.gov/pubmed/28382032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.00493
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