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Application of an E. coli signal sequence as a versatile inclusion body tag

BACKGROUND: Heterologous protein production in Escherichia coli often suffers from bottlenecks such as proteolytic degradation, complex purification procedures and toxicity towards the expression host. Production of proteins in an insoluble form in inclusion bodies (IBs) can alleviate these problems...

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Bibliografiske detaljer
Udgivet i:Microb Cell Fact
Main Authors: Jong, Wouter S. P., Vikström, David, Houben, Diane, van den Berg van Saparoea, H. Bart, de Gier, Jan-Willem, Luirink, Joen
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5359840/
https://ncbi.nlm.nih.gov/pubmed/28320377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-017-0662-4
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