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Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.

A target nucleic acid sequence can be replicated (amplified) exponentially in vitro under isothermal conditions by using three enzymatic activities essential to retroviral replication: reverse transcriptase, RNase H, and a DNA-dependent RNA polymerase. By mimicking the retroviral strategy of RNA rep...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Guatelli, J C, Whitfield, K M, Kwoh, D Y, Barringer, K J, Richman, D D, Gingeras, T R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1990
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53586/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2308948/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.5.1874
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