Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.

A target nucleic acid sequence can be replicated (amplified) exponentially in vitro under isothermal conditions by using three enzymatic activities essential to retroviral replication: reverse transcriptase, RNase H, and a DNA-dependent RNA polymerase. By mimicking the retroviral strategy of RNA rep...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Principais autores:	Guatelli, J C, Whitfield, K M, Kwoh, D Y, Barringer, K J, Richman, D D, Gingeras, T R
Formato:	Artigo
Idioma:	Inglês
Publicado:	National Academy of Sciences 1990
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53586/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2308948/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.5.1874
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