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Total chemical synthesis of proteins without HPLC purification
The total chemical synthesis of proteins is a tedious and time-consuming endeavour. The typical steps involve solid phase synthesis of peptide thioesters and cysteinyl peptides, native chemical ligation (NCL) in solution, desulfurization or removal of ligation auxiliaries in the case of extended NCL...
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| Gepubliceerd in: | Chem Sci |
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| Hoofdauteurs: | , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Royal Society of Chemistry
2016
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5355786/ https://ncbi.nlm.nih.gov/pubmed/28451120 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6sc01883a |
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