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Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

The methanol-regulated alcohol oxidase promoter (P(AOX1)) of Pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. Although increasing gene dosage is one of the most common strategies to increase recombinant protein productivities, the incre...

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Detaylı Bibliyografya
Yayımlandı:Sci Rep
Asıl Yazarlar: Cámara, Elena, Landes, Nils, Albiol, Joan, Gasser, Brigitte, Mattanovich, Diethard, Ferrer, Pau
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Nature Publishing Group 2017
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5353721/
https://ncbi.nlm.nih.gov/pubmed/28295011
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep44302
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