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ThermoAlign: a genome-aware primer design tool for tiled amplicon resequencing

Isolating and sequencing specific regions in a genome is a cornerstone of molecular biology. This has been facilitated by computationally encoding the thermodynamics of DNA hybridization for automated design of hybridization and priming oligonucleotides. However, the repetitive composition of genome...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Francis, Felix, Dumas, Michael D., Wisser, Randall J.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5353602/
https://ncbi.nlm.nih.gov/pubmed/28300202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep44437
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