Multiplex PCR Targeting tpi (Triose Phosphate Isomerase), tcdA (Toxin A), and tcdB (Toxin B) Genes for Toxigenic Culture of Clostridium difficile

A multiplex PCR toxigenic culture approach was designed for simultaneous identification and toxigenic type characterization of Clostridium difficile isolates. Three pairs of primers were designed for the amplification of (i) a species-specific internal fragment of the tpi (triose phosphate isomerase...

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Bibliographic Details

Published in:	J Clin Microbiol
Main Authors:	Lemee, Ludovic, Dhalluin, Anne, Testelin, Sabrina, Mattrat, Marie-Andre, Maillard, Karine, Lemeland, Jean-François, Pons, Jean-Louis
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2004
Subjects:	Bacteriology
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535266/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15583303/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.12.5710-5714.2004
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