Multiplex PCR Targeting tpi (Triose Phosphate Isomerase), tcdA (Toxin A), and tcdB (Toxin B) Genes for Toxigenic Culture of Clostridium difficile

A multiplex PCR toxigenic culture approach was designed for simultaneous identification and toxigenic type characterization of Clostridium difficile isolates. Three pairs of primers were designed for the amplification of (i) a species-specific internal fragment of the tpi (triose phosphate isomerase...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Lemee, Ludovic, Dhalluin, Anne, Testelin, Sabrina, Mattrat, Marie-Andre, Maillard, Karine, Lemeland, Jean-François, Pons, Jean-Louis
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2004
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535266/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15583303/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.12.5710-5714.2004
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