Development of a Rapid PCR Method Using the Insertion Sequence IS1203 for Genotyping Shiga Toxin-Producing Escherichia coli O157

We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-fac...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Clin Microbiol
Prif Awduron: Suzuki, Masahiro, Matsumoto, Masakado, Hata, Mami, Takahashi, Masao, Sakae, Kenji
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology (ASM) 2004
Pynciau:
Bacteriology
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535262/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15583266/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.12.5462-5466.2004
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