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Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157

Real-time PCR assays have been applied for the detection and quantification of pathogens in recent years. In this study two combinations of primers and fluorescent probes were designed according to the sequences of the rfb(Escherichia coli O157) and stx(2) genes. Analysis of 217 bacterial strains de...

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Bibliografski detalji
Glavni autori: Hsu, Ching-Fang, Tsai, Tsung-Yu, Pan, Tzu-Ming
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151888/
https://ncbi.nlm.nih.gov/pubmed/15956382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.6.2668-2673.2005
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