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Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157

Real-time PCR assays have been applied for the detection and quantification of pathogens in recent years. In this study two combinations of primers and fluorescent probes were designed according to the sequences of the rfb(Escherichia coli O157) and stx(2) genes. Analysis of 217 bacterial strains de...

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Detalhes bibliográficos
Main Authors: Hsu, Ching-Fang, Tsai, Tsung-Yu, Pan, Tzu-Ming
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151888/
https://ncbi.nlm.nih.gov/pubmed/15956382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.6.2668-2673.2005
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