Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Apart from their essential role in generating ATP, mitochondria also act as local calcium (Ca(2+)) buffers to tightly regulate intracellular Ca(2+) concentration. To do this, mitochondria utilize the electrochemical potential across their inner membrane (ΔΨ(m)) to sequester Ca(2+). The influx of Ca(...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: McKenzie, Matthew, Lim, Sze C., Duchen, Michael R.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2017
Fag:
Cellular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5352290/
https://ncbi.nlm.nih.gov/pubmed/28190045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55166
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