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Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
Apart from their essential role in generating ATP, mitochondria also act as local calcium (Ca(2+)) buffers to tightly regulate intracellular Ca(2+) concentration. To do this, mitochondria utilize the electrochemical potential across their inner membrane (ΔΨ(m)) to sequester Ca(2+). The influx of Ca(...
Guardat en:
| Publicat a: | J Vis Exp |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
MyJove Corporation
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5352290/ https://ncbi.nlm.nih.gov/pubmed/28190045 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55166 |
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