Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Protein-protein interactions are involved in thousands of cellular processes and occur in distinct spatial context. Traditionally, co-immunoprecipitation is a popular technique to detect protein-protein interactions. Subsequent Western blot analysis is the most common method to visualize co-immunopr...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Zhu, Xinzhou, Zelmer, Andrea, Wellmann, Sven
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2017
Subjects:
Biochemistry
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5352272/
https://ncbi.nlm.nih.gov/pubmed/28117799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55218
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