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Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Here we present a method for establishing multiple component cell culture hydrogels for in vitro lung cell culture. Beginning with healthy en bloc lung tissue from porcine, rat, or mouse, the tissue is perfused and submerged in subsequent chemical detergents to remove the cellular debris. Histologic...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Vis Exp
Prif Awduron: Link, Patrick A., Pouliot, Robert A., Mikhaiel, Nabil S., Young, Bethany M., Heise, Rebecca L.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MyJove Corporation 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5352266/
https://ncbi.nlm.nih.gov/pubmed/28117788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55094
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