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Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Here we present a method for establishing multiple component cell culture hydrogels for in vitro lung cell culture. Beginning with healthy en bloc lung tissue from porcine, rat, or mouse, the tissue is perfused and submerged in subsequent chemical detergents to remove the cellular debris. Histologic...

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Dades bibliogràfiques
Publicat a:J Vis Exp
Autors principals: Link, Patrick A., Pouliot, Robert A., Mikhaiel, Nabil S., Young, Bethany M., Heise, Rebecca L.
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5352266/
https://ncbi.nlm.nih.gov/pubmed/28117788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55094
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