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Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
Here we present a method for establishing multiple component cell culture hydrogels for in vitro lung cell culture. Beginning with healthy en bloc lung tissue from porcine, rat, or mouse, the tissue is perfused and submerged in subsequent chemical detergents to remove the cellular debris. Histologic...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5352266/ https://ncbi.nlm.nih.gov/pubmed/28117788 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55094 |
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