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Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
We demonstrate a useful method for quantifying autophosphorylation of purified bacterial histidine kinases. Histidine kinases are known for their involvement in two-component signal transduction, a ubiquitous system through which bacteria sense and respond to environmental stimuli. Two-component sig...
Gespeichert in:
| Veröffentlicht in: | J Vis Exp |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
MyJove Corporation
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5352214/ https://ncbi.nlm.nih.gov/pubmed/28117829 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55129 |
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