Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces

16S rRNA gene-targeted group-specific primers were designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster. To monitor the predominant bacteria in human feces by real-time PCR, we used these specific primers together with four set...

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Detalhes bibliográficos
Main Authors: Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Takada, Toshihiko, Tanaka, Ryuichiro
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Microbial Ecology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC535136/
https://ncbi.nlm.nih.gov/pubmed/15574920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7220-7228.2004
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