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Overestimation of the Abundance of Sulfate-Reducing Bacteria in Human Feces by Quantitative PCR Targeting the Desulfovibrio 16S rRNA Gene

The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting...

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Autores principales: Christophersen, C. T., Morrison, M., Conlon, M. A.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3126453/
https://ncbi.nlm.nih.gov/pubmed/21460115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02851-10
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