Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

Ultrahigh-throughput screening (uHTS) techniques can identify unique functionality from millions of variants. To mimic the natural selection mechanisms that occur by compartmentalization in vivo, we developed a technique based on single-cell encapsulation in droplets of a monodisperse microfluidic d...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Terekhov, Stanislav S., Smirnov, Ivan V., Stepanova, Anastasiya V., Bobik, Tatyana V., Mokrushina, Yuliana A., Ponomarenko, Natalia A., Belogurov, Alexey A., Rubtsova, Maria P., Kartseva, Olga V., Gomzikova, Marina O., Moskovtsev, Alexey A., Bukatin, Anton S., Dubina, Michael V., Kostryukova, Elena S., Babenko, Vladislav V., Vakhitova, Maria T., Manolov, Alexander I., Malakhova, Maja V., Kornienko, Maria A., Tyakht, Alexander V., Vanyushkina, Anna A., Ilina, Elena N., Masson, Patrick, Gabibov, Alexander G., Altman, Sidney
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5347554/
https://ncbi.nlm.nih.gov/pubmed/28202731
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1621226114
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