Harnessing molecular motors for nanoscale pulldown in live cells

Protein–protein interactions (PPIs) regulate assembly of macromolecular complexes, yet remain challenging to study within the native cytoplasm where they normally exert their biological effect. Here we miniaturize the concept of affinity pulldown, a gold-standard in vitro PPI interrogation technique...

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Dades bibliogràfiques
Publicat a:Mol Biol Cell
Autors principals: Bird, Jonathan E., Barzik, Melanie, Drummond, Meghan C., Sutton, Daniel C., Goodman, Spencer M., Morozko, Eva L., Cole, Stacey M., Boukhvalova, Alexandra K., Skidmore, Jennifer, Syam, Diana, Wilson, Elizabeth A., Fitzgerald, Tracy, Rehman, Atteeq U., Martin, Donna M., Boger, Erich T., Belyantseva, Inna A., Friedman, Thomas B.
Format: Artigo
Idioma:Inglês
Publicat: The American Society for Cell Biology 2017
Matèries:
Articles
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5341729/
https://ncbi.nlm.nih.gov/pubmed/27932498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E16-08-0583
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