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Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent

Photobleaching remains a limiting factor in superresolution fluorescence microscopy. This is particularly true for stimulated emission depletion (STED) and reversible saturable/switchable optical fluorescence transitions (RESOLFT) microscopy, where adjacent fluorescent molecules are distinguished by...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Göttfert, Fabian, Pleiner, Tino, Heine, Jörn, Westphal, Volker, Görlich, Dirk, Sahl, Steffen J., Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5338502/
https://ncbi.nlm.nih.gov/pubmed/28193881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1621495114
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