Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme.
How easy is it to improve the catalytic power of an enzyme? To address this question, the gene encoding a sluggish mutant triose-phosphate isomerase (D-glyceraldehyde-3-phosphate ketol-isomerase, EC 5.3.1.1) has been subjected to random mutagenesis over its whole length by using "spiked" oligonucleo...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53332/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1967829/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.2.696 |
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