Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP

BACKGROUND: While NGS allows rapid global detection of transcripts, it remains difficult to distinguish ncRNAs from short mRNAs. To detect potentially translated RNAs, we developed an improved protocol for bacterial ribosomal footprinting (RIBOseq). This allowed distinguishing ncRNA from mRNA in EHE...

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Gepubliceerd in:BMC Genomics
Hoofdauteurs: Neuhaus, Klaus, Landstorfer, Richard, Simon, Svenja, Schober, Steffen, Wright, Patrick R., Smith, Cameron, Backofen, Rolf, Wecko, Romy, Keim, Daniel A., Scherer, Siegfried
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BioMed Central 2017
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Research Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5331693/
https://ncbi.nlm.nih.gov/pubmed/28245801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-017-3586-9
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