Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP

BACKGROUND: While NGS allows rapid global detection of transcripts, it remains difficult to distinguish ncRNAs from short mRNAs. To detect potentially translated RNAs, we developed an improved protocol for bacterial ribosomal footprinting (RIBOseq). This allowed distinguishing ncRNA from mRNA in EHE...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Neuhaus, Klaus, Landstorfer, Richard, Simon, Svenja, Schober, Steffen, Wright, Patrick R., Smith, Cameron, Backofen, Rolf, Wecko, Romy, Keim, Daniel A., Scherer, Siegfried
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5331693/
https://ncbi.nlm.nih.gov/pubmed/28245801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-017-3586-9
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