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Evidence that insulin and isoprenaline activate the cGMP-inhibited low-Km cAMP phosphodiesterase in rat fat cells by phosphorylation.

Incubation of intact rat fat cells with maximally effective concentrations of insulin (1 nM, 12 min) or isoprenaline (300 nM, 3 min) increased particulate cGMP- and cilostamide-inhibited, low-Km cAMP phosphodiesterase (cAMP-PDE) activity by about 50% and 100%, respectively. In 32P-labeled cells, the...

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Autors principals: Degerman, E, Smith, C J, Tornqvist, H, Vasta, V, Belfrage, P, Manganiello, V C
Format: Artigo
Idioma:Inglês
Publicat: 1990
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC53299/
https://ncbi.nlm.nih.gov/pubmed/2153956
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