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Genome-wide profiling of RNA polymerase transcription at nucleotide resolution in human cells with native elongating transcript sequencing

Many features of gene transcription in human cells remain unclear, mainly due to a lack of quantitative approaches to follow genome transcription with nucleotide precision in vivo. Here we present a robust genome-wide approach to study RNA polymerase (Pol) II-mediated transcription in human cells at...

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Vydáno v:Nat Protoc
Hlavní autoři: Mayer, Andreas, Churchman, L. Stirling
Médium: Artigo
Jazyk:Inglês
Vydáno: 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5326677/
https://ncbi.nlm.nih.gov/pubmed/27010758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.047
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