GTP-binding Ypt1 protein and Ca2+ function independently in a cell-free protein transport reaction.

The 21-kDa GTP-binding Ypt1 protein (Ypt1p) is required for protein transport from the endoplasmic reticulum to the Golgi complex in yeast extracts. Ypt1 antibodies block transport; this inhibition is alleviated by competition with excess purified Ypt1p produced in bacteria. Furthermore, extracts of...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Baker, D, Wuestehube, L, Schekman, R, Botstein, D, Segev, N
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53262/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2104983/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.1.355
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