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GTP-binding Ypt1 protein and Ca2+ function independently in a cell-free protein transport reaction.

The 21-kDa GTP-binding Ypt1 protein (Ypt1p) is required for protein transport from the endoplasmic reticulum to the Golgi complex in yeast extracts. Ypt1 antibodies block transport; this inhibition is alleviated by competition with excess purified Ypt1p produced in bacteria. Furthermore, extracts of...

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Bibliographische Detailangaben
Hauptverfasser: Baker, D, Wuestehube, L, Schekman, R, Botstein, D, Segev, N
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1990
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC53262/
https://ncbi.nlm.nih.gov/pubmed/2104983
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