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Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display with additional random sequences in the terminal translated regions

In vitro display technologies such as mRNA and cDNA display are powerful tools to create and select functional peptides. However, in some cases, efficiency of mRNA-protein fusion is very low, which results in decreased library size and lower chance of successful selection. In this study, to improve...

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Publicat a:Biophys Physicobiol
Autors principals: Takahashi, Kazuki, Sunohara, Masato, Terai, Takuya, Kumachi, Shigefumi, Nemoto, Naoto
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society of Japan (BSJ) 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5325054/
https://ncbi.nlm.nih.gov/pubmed/28275529
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2142/biophysico.14.0_23
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