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Increasing the library size in cDNA display by optimizing purification procedures

BACKGROUND: The library size is critical for selection in evolutionary molecular engineering (directed evolution). Although cDNA display has become a promising in vitro display technology by overcoming the instability of mRNA display, it is hindered by low yields. In this study, we improved the yiel...

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Bibliographische Detailangaben
Hauptverfasser: Mochizuki, Yuki, Kumachi, Shigefumi, Nishigaki, Koichi, Nemoto, Naoto
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3680162/
https://ncbi.nlm.nih.gov/pubmed/23697943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1480-9222-15-7
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