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Refined protocol for generating monoclonal antibodies from single human and murine B cells

Generating monoclonal antibodies from single B cells is a valuable tool for characterizing the specificity and functional properties of humoral responses. We and others developed protocols that have facilitated major advances in our understanding of B cell development, tolerance, and effector respon...

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Detalhes bibliográficos
Publicado no:J Immunol Methods
Main Authors: Ho, Irvin Y., Bunker, Jeffrey J., Erickson, Steven A., Neu, Karlynn E., Huang, Min, Cortese, Mario, Pulendran, Bali, Wilson, Patrick C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5322767/
https://ncbi.nlm.nih.gov/pubmed/27600311
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2016.09.001
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