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Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins

We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed fret donor, monomeric cyan-excitable red fluorescent protein (mCyRFP), which has a large stokes shift and a monoexponential...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Laviv, Tal, Kim, Benjamin B, Chu, Jun, Lam, Amy J, Lin, Michael Z, Yasuda, Ryohei
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5322478/
https://ncbi.nlm.nih.gov/pubmed/27798609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4046
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