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Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins
We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed fret donor, monomeric cyan-excitable red fluorescent protein (mCyRFP), which has a large stokes shift and a monoexponential...
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| Udgivet i: | Nat Methods |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5322478/ https://ncbi.nlm.nih.gov/pubmed/27798609 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4046 |
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